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It is similar to classical melting curve analysis, but provides far more information for a wider range of applications. HRM characterizes double-stranded PCR products based on their dissociation (melting) behavior. High-resolution melting analysis (HRM) is a closed-tube, post-PCR analysis that has raised enormous scientific interest. JOE, VIC, HEX, TET, MAX, CAL Fluor Gold 540, Yakima Yellow In addition, the software allows you to create new excitation/detection wavelength combinations, which means that the Rotor-Gene Q is compatible with dyes you may use in the future. With up to 6 channels spanning UV to infrared wavelengths, the cycler delivers the widest optical range currently available (see table). Whether your assay is based on intercalating dyes such as SYBR ® Green, probes such as hydrolysis (TaqMan®), hybridization (FRET), Scorpion probes, or other multiplex chemistries, the Rotor-Gene Q meets your requirements. Unrivaled optical range enables multiple applications
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Uniform detection eliminating the need for ROX reference dye.These are unavoidable in traditional block-based instruments due to temperature gradients across the block and multiple, complex optical pathways. It also eliminates sample-to-sample variations and edge effects. This thermal and optical uniformity results in sensitive, precise, and fast real-time PCR analysis (see figure “ Precise real-time PCR analysis“).
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When each tube aligns with the detection optics, the sample is illuminated and the fluorescent signal is rapidly collected from a single, short optical pathway. Each tube spins in a chamber of moving air, keeping all samples at precisely the same temperature during rapid thermal cycling. The unique centrifugal rotary design of the Rotor-Gene Q makes it the most precise and versatile real-time PCR cycler currently available (see figure “ Cross-section of the Rotor-Gene Q“). Unique rotary design for outstanding performance